The Effects of Oil and Oil + Nicotine in Vaporizer Pens (E-Cigarettes) on the Cell Behavior of Undifferentiated Human Mesenchymal Stem, and Differentiated Human Foreskin Fibroblast

                                           http://austinpublishinggroup.com/biomedical-engineering/

Electroniccigarettes, also known as vaporizable pens, are a fairly new product on the market advertised as a safer alternative to smoking cigarettes or as a method to quit smoking entirely. Unlike traditional cigarettes, vaporizable pens do not burn tobacco but instead employ a battery-powered heating element that vaporizes a liquid oil to emulate the sensation of smoking. Various epidemiological studies reveal a considerable correlation between smoking or the use of smokeless tobacco substances and the prevalence of emphysema, heart disease, stroke and various cancers. Despite their growing popularity, the biological consequences of the newly popular nicotine oils inhaled from the vaporizable pens on human health are not well known and have not been heavily researched.

Nicotine,the key constituent in cigarettes and nicotine containing vaporizable oil, has been shown to reduce cell viability and inhibit cellular proliferation of fibroblast cells in vitro. Fibroblast cells are connective tissue cells that are responsible for secreting collagen proteins. As a derivative of mesenchymal origin, they are inhabitants of the Extracellular Matrix (ECM) of connective tissues and are responsible for secreting proteins used to maintain tissue structure. Fibroblasts play a vital role in the final stages of wound healing and use in many novel applications in tissue engineering. Mesenchymal stem cells (MSCs) have been investigated for their potential use in cell-based therapies in clinical management of difficult wounds and therapeutic dermatology. While fibroblasts cells are traditionally involved in wound healing, mesenchymal stem cells exhibit fibroblast like plastic-adherent morphology and have the capacity for multipotent differentiation in vitro and in vivo.

Data currentlysuggests that MSC differentiation pathways are regulated through dynamic interactions with the Extracellular Matrix (ECM). The ECM is a dynamic network of macromolecules present within all mammalian tissues that provides not only the physical scaffolding between cells but also essential biochemical cues to initiate differentiation and promote cell communication amongst