http://austinpublishinggroup.com/microbiology/fulltext/ajm-v2-id1010.php
The rapid spread of acquired
Metallo-Beta-Lactamases (MBL) among major Gram-negative pathogens is an
emerging threat and a matter of concern worldwide as it results into fewer
therapeutic options for the treatment. Therefore, this study was undertaken to
determine the prevalence of MBL producing Gram-negative bacteria isolated from
different clinical samples.
A total of 490 samples were analyzed, at the
Microbiology Department of Shahid Gangalal National Heart Centre (SGNHC),
Bansbari, Kathmandu from December 2013 to June 2014, for routine culture and
antibiotic susceptibility testing. MBL detection was done by Imipenem-EDTA
Combined Disc Test.
Out of 490 samples analyzed, 107 showed
positive growth. Fortytwo percent of the Gram-negative isolates were Multi Drug
Resistant (MDR). Among 107 Gram-negative isolates, 66 ceftazidime resistant
isolates were screened for MBL production of which 9 (13.6%) were found to be
MBL positive. All MBL positive isolates were Pseudomonasaeruginosa. None other
Gramnegative bacteria were found to produce MBL. Prevalence of MBL producing P.
aeruginosa was 20% and
all the isolates were MDR. All the MBL producing P.
aeruginosa were isolated
from hospitalized patients.
This study showed MBL production in a
considerable number of P. aeruginosa isolates with
MDR phenotypes. There is a need to track the detection of MBL producers and
judicious use of carbapenems is necessary to prevent the further spread of
these organisms.
AST: Antibiotic Susceptibility Test; ATCC:
American Type Culture Collection; CLSI: Clinical Laboratory Standard Institute;
EDTA: Ethylene-Diamine-Tetraacetic Acid; ESBL: Extended-
Spectrum-Beta-Lactamase; ICU: Intensive Care Unit; MBL: Metallo- Beta-Lactamase
(Metallo-β-lactamase); MBLs: Metallo-Beta- Lactamases; MDR: Multi-Drug
Resistance; SPSS: Statistical Package for Social Science; TUTH: Tribhuvan
University Teaching Hospital; ZOI: Zone of Inhibition.
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