Despite of the common efforts in biodefense around the world the problem
of high dangerous pathogens distribution, especially under the threat of
terrorist attacks, is still urgent. Among the biotoxins that could be used for
dispersion, leading experts identify large number of high pathogen
microorganisms of category A and B, such as well-known pathogens: Bacillus
anthracis, Salmonella, Escherichia etc.
The most important problem in the
struggle against biohazard is high effective defense of civil population. Such
effective defense can be provided using safe drugs, which are effective against
wide range of biotoxins.
Modern antimicrobial preparations and vaccines don’t possess by
wide range of activity against different classes of pathogens. So, the search
and development of new high effective antimicrobial substances with high
effectiveness against wide spectra of high pathogen microorganisms - causative
agents of most dangerous human and animals’ diseases is topical problem today.
Metal nanoparticles are possessed by high potential in this area. The main goal of presented work was antimicrobial activity
investigation of the synthesized bismuth nanoparticles against wide spectra of
pathogen microorganisms - potential causative agents of high dangerous human
and animals’ diseases.
Spherical Bismuth Nanoparticles
(BiNP) have been synthesized by the method of chemical condensation in water
medium. The concentration of obtained BiNP was 77.5 mg/ml by metal. The shape Transmission
Electron Microscopy (TEM) (JEM-1230 «JEOL LTD», Japan). The method of
energy-dispersive X-ray spectroscopy has been used for chemical composition’s
X-ray microanalysis of the synthesized nanoparticles (IETEM 250 with detector
X-Max 80, Oxford Instruments Analytical, UK for JEM-1230).iNP biosafety level has been estimated in vitro using
parameters of cytotoxicity, genotoxicity, mutagenicity and biochemical markers
according to the Guidelines «Safety assessment of medical nanopreparations.
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