http://austinpublishinggroup.com/biomolecules/\
Cyclodextringlycosyltransferase (CGTase) is an extracellular enzyme commonly produced by
alkaliphilic bacilli. In this article, we report one-step synthesis of silver
nanoparticles (Ag-NPs) through a green method using CGTase as both reducing and
stabilizing agents. Structural, morphological and optical properties of the Ag-NPs
were characterized using X-Ray Diffraction (XRD), Transmission Electron
Microscopy (TEM) and UV-Vis spectroscopy. The formations of Ag-NPs were
confirmed with an absorbance band centered at 450nm. A TEM image displayed that
the nanoparticles are spherical in shape with size ranging from 10 to 25nm. The
XRD pattern showed that the nanoparticles were well arranged in the right
crystalline system. The bio-formed nanoparticles are expected to have prominent
uses in pharmaceutical and biomedical areas.
Researchersin the field of nanotechnology are turning to ‘Nature’ to provide inspiration,
for stimulating and innovative approaches of nano-production. Synthesizing new
metallic nanoparticles based on the notion of green nanotechnology is obtaining
momentum. Green nanotechnology mixes the principles of green engineering and
green chemistry to produce safe and eco-friendly nanoparticles, which do not
use toxic substances in their synthesis procedure. The synthesis of
nanoparticles of noble metals, such as Ag-NPs, is of excessive interest because
of their unique characteristics. Manipulation of their shape and size creates
unique properties which have potential applications in biomedical uses such as
antibacterial, anti-HIV activity, controlling plant pathogens and as a
biosensor and catalyst.
Currentchemical and physical techniques for the production of Ag-NPs use hazardous
substances for example hydrazine, Dimethyl formamide (DMF) and Sodium
borohydride, as reducing agents, and may also need to use costly instruments.
These approaches produce Ag-NP efficiently, nevertheless downstream processing
to distinct nanoparticles from the toxic compounds is costly and time
consuming. Existence of even a slight trace of toxic compounds makes these
Ag-NPs incompatible for pharmaceutical and biomedical applications.
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