Hydrochlorideis a long acting α-adrenergic agonist that causes elevation of systemic blood
pressure, accompanied by a reduction in heart rate. Midodrine is a pro-drug of
desglymidodrine (DMAE), developed by the attachment of the amino acid approach
glycine to the functional amine of DMAE. It is therapeutically used, as a
racemic (rac) mixture, for the treatment of orthostatic hypotension. The
pro-drug Midodrine is primarily converted into its active metabolite
desglymidodrine after oral administration, mainly in the liver and in the
systemic circulation by unknown peptidases. After oral administration,
Midodrine is rapidly absorbed. The plasma levels of the pro-drug peak after
about half an hour, and decline with a half- life of approximately 25 minutes,
while the metabolite reaches peak blood concentrations about 1 to 2 hours after
a dose of Midodrine and has a half-life of about 3 to 4 hours. Until now, the
metabolism of Midodrine has not been extensively studied. Midodrine
was developed by an amino-acid approach through the glycine promoiety
attachment to the functional amine of desglymidodrine. Midodrine is a substrate
for the intestinal H+ -coupled
peptide transporter 1 (hPEPT1). This carrier mediated transport raises the
bioavailability of Midodrine Hydrochloride to 93% when compared with 50% for
desglymidodrine.
Asuitable and validated method should be vacant for the drug delivery system for
analysis of bulk drug, for release dissolution studies and estimation of drug
in biological samples. The literature survey acknowledges that various methods
for the determination of Midodrine hydrochlorides are noted. Some methods have
been developed based on radioisotope-labeled techniques, High Performance
Liquid Chromatography (HPLC) with fluorescence and Ultraviolet (UV) detection,
and the Capillary Electrophoresis (CE). Hence, our study reports a simple,
precise and economical UV- Spectrophotometric method for estimation of
Midodrine Hydrochloride in bulk and tablet formulation. The method was
validated according to ICH guidelines.
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