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Monday, 16 October 2017

Effect of Ginseng on the Testis of Subclinical Hypothyroidism Model in Adult Male Albino Rat



Hyperstimulation of follicular cells using a goitrogen Propylthiouracil (PTU) caused Subclinical Hypothyroidism (SCH) that was associated with disturbances in adult male testicular functions. This work was carried out to study the probable relationship between follicular cells in drug-induced hypothyroidism caused by PTU and consequently its effect on testicular tissue and the possible modulating effect of ginseng. The present work was carried out on 40 adult male albino rats divided into four groups. Group I was the control group; Group II (Ginseng treated); Group III (SCH group) rats and Group IV was the treated group. Testes and thyroid glands were studied. Our results revealed the protective role of Ginseng on testis and thyroid tissue of experimentally induced hypothyroidism rat model.


Thyroid hormone plays an important role in testicular development and function. It has been established that Triiodothyronine (T3) regulates the maturation and growth of the testis, controlling Sertoli cells and Leydig cell proliferation and differentiation during testicular development in rats and other species. Subclinical Hypothyroidism (SCH) could be considered as an elevated serum thyrotropic stimulating hormone (TSH) level associated with normal Thyroxine (T4) and Triiodothyronine (T3) levels with no clinical symptoms of hypothyroidism. This thyroid dysfunction occurs in 4-20% of the adult population, the possibility of SCH progression to clinical hypothyroidism is around 7% [2]. Hypothyroidism extremely affects reproductive functions including fertility, pregnancy and postnatal development in human and rat [3]. Rat SCH model was induced mostly by administration of antithyroid drug mostly Propylthiouracil (PTU) and Methimazole (MMI). PTU is a thiouracil-derived drug which can be used to treat hyperthyroidism through lowering the amount of thyroid hormone produced by the thyroid gland [5]. PTU inhibits conversion of T4 to T3 causing hypothyroidism [6]. Ginseng is considered as an aromatic herb that is commonly used in herbal medicine. It contains saponins and high iodine content. Ginseng saponins, which are known as ginsenosides, have been studied to be responsible for its medicinal effects, specifically anticancer, antihypertensive, antidiabetic, and antistress effects. Ginseng benefits all the endocrine system, and therefore the thyroid gland. As ginseng markedly increased epididymal sperm count, motility and hyperactivation in mice. So, we studied its effect on the testis and thyroid of SCH model.

Forty adult Westar male albino rats were used in this experiment, each weighting 150-200gm. Food and water were provided ad libitum and the rats were left for 7 days for acclimatization before use in the Anatomy Department, Faculty of medicine, Menoufia University. All aspects of animal care and treatment were carried out according to the local guidelines of the ethical committee for animal research.

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