Inhibitor Targeting METTL3 Partially Reverses Damage Associated with Pulmonary Arterial Hypertension in Mouse and Cell Models

Background: Pulmonary arterial hypertension is a potentially fatal condition with no effective cure. Recent studies have shown that progressively increasing pulmonary vascular resistance and irreversible pulmonary vascular remodeling are key factors contributing to pulmonary hypertension. Methyltransferase like 3 (METTL3), a key N6-Methyladenosine (m6A) methyltransferase, it has been shown that elevated levels of METTL3 expression is a risk factor for pulmonary hypertension, Elevated levels of METTL3 contribute to vascular remodeling in the lungs, resulting in increased pulmonary artery pressure and right ventricular hypertrophy. Methods: To obtain a model of pulmonary hypertension, we placed C57BL/6 male mice in a 500-liter ventilated room with a 10% oxygen concentration for four weeks. After mice were placed in a hypoxic incubator for two weeks, they were started on intraperitoneal injections of STM2457 once a day for two weeks, after which right ventricular pressure was measured using a Miller catheter, right ventricular remodeling is determined using hematoxylin-eosin staining, right ventricular hypertrophy was assessed using the right ventricular/left ventricular + septum (RV/LV+S) ratio and relative expression of TNF-α, IL-1β, IL-6 protein by western blot. The impact of STM2457 on Human Pulmonary Artery Smooth Muscle Cells (HPASMCs) under hypoxia was examined by evaluating their viability, proliferation, migration and the expression of IL-1β, IL-6, and TNF-α proteins. Results: In mouse models of hypoxic PAH, METTL3 expression was significantly increased and pulmonary vascular remodeling. Treatment with STM2457 significantly decreased right ventricular pressure and hypertrophy, and inhibited molecules associated with the inflammation signaling pathway. Additionally, the inhibition of METTL3 by STM2457 attenuated hypoxia-induced proliferation and migration of HPASMCs, while further inhibiting molecules associated with the inflammation signaling pathway. Conclusions: Based on the results obtained above, we are confident that treatment with STM2457 targeting METTL3 significantly reduces damage in PAH model mice and cells.

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Corresponding author: Hui Li**

Full Length Article: Inhibitor Targeting METTL3 Partially Reverses Damage Associated with Pulmonary Arterial Hypertension in Mouse and Cell Models

Journal: Austin Journal of Nutrition & Metabolism

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A Simplified Splint Tubing Technique of Heterotopic Heart Transplantation in Rat

Background: Heterotopic Heart Transplantation (HHT) in rats has been established for the study of transplantation immunology. Our goal was to describe a Splint Tubing Technique (STT) for cervical HHT in rats. Methods: Wistar-Furth rats were performed in HHT using STT and Suture Technique (ST). With STT, the surgical procedure connected with the recipient external jugular vein (EJV), Common Carotid Artery (CCA) to the donor Pulmonary Artery (PA), Ascending Aorta (AA) using an 18G and 20G cuff, respectively. The surgical success rate and operation time was recorded. Graft function was assessed by pulse palpation and echocardiography. The cardiac pathology was analyzed by HE staining. TUNEL and western blotting were used to measure apoptosis in each rat. Results: Averaged total operation time using ST was much longer than STT. The success rate of STT was 83.3%, which showed significantly higher than ST (55.0%). HE staining of STT group showed the same acute rejection compared to ST group. Same tendency of apoptosis in two groups was detected by TUNEL and western blotting. Conclusions: Our simplified STT for HHT in rats is an easy, convenient, stable, and reliable method. It simplified the rat HHT procedure, shortened operation time, reduced surgical difficulty, improved technical success rate.

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Corresponding author: Xuechao Yang**

Full Length Article: A Simplified Splint Tubing Technique of HeterotopicHeart Transplantation in Rat

Journal: Thrombosis & Haemostasis: Research

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The Effect of Nitrogen and NPS Fertilizer Rates and Inter-row Spacing on the Growth and Yield of Head Cabbage (Brassica oleracea L.) under Irrigation in Bako, Western Oromia, Ethiopia

Head cabbage is a popular leafy vegetable consumed all over the world. However, a number of factors, such as diminished soil fertility and poor agronomic practices, frequently restrict the crop’s production and productivity. A study was conducted at Bako Agricultural Research Center, under surface irrigation, during the cropping seasons of 2022 and 2023 for two years, to determine the effects of three levels of inter-row spacing, Nitrogen from urea, and Phosphorus from (NPS), were laid out in a randomized block design with three replications in a 3x3x3 factorial arrangement. The results analysis of variance indicated that the inter-row spacing of 55 cm had a substantial effect on the marketable yield (27483 kg ha-1) and total yield, (31842 kg ha-1). Likewise, 69 N ha-1 fertilizer rates from urea gave the highest marketable yield of 27861 kg ha-1 and total cabbage yield of 31824 kg ha-1. The partial budget analysis across year showed the highest net benefit of, 274720.0 birr obtained. Therefore, application 69 N ha-1 fertilizers and 100 kg ha-1 NPS with inter-row spacing of 55 cm under irrigation can be recommended for the production of head cabbage in study area and similar agroecology ecology based on the study’s findings

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Corresponding author: Mamo Mekonnen Feyanbule*

Full Length Article: The Effect of Nitrogen and NPS Fertilizer Rates andInter-row Spacing on the Growth and Yield of HeadCabbage (Brassica oleracea L.) under Irrigation in Bako,Western Oromia, Ethiopia

Journal: Annals of Agricultural & Crop Sciences

Disclaimer: This content is not owned or created by us. It has been sourced from the respective site, Austin Publishing Group, and is intended purely for study and educational purposes. All rights belong to the original authors and publishers.

Advancing Laparoscopic Cholecystectomy, A Few Messages

Laparoscopic cholecystectomy is a safe and common procedure, but it can be associated with some complications. These complications may occur during the surgery or in the following days or weeks. We will highlight some complications or risks of laparoscopic cholecystectomy through three clinical images while explaining the preventive measures

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Corresponding author: Javier Espina Hellín*

Full Length Article: Optimizing Drug Distribution Logistics in the Community of Madrid

Journal: Journal of Family Medicine

Disclaimer: This content is not owned or created by us. It has been sourced from the respective site, Austin Publishing Group, and is intended purely for study and educational purposes. All rights belong to the original authors and publishers.

Optimizing Drug Distribution Logistics in the Community of Madrid

Health-related services, and specifically drugs, are one of the most important economic activities in modern societies. Drugs, as a final medical product, constitute the basis for improving the quality of health services. In this article, we propose as a general working hypothesis that better rationalization in the logistics distribution of drugs, through a combination of an optimal location of distribution centers, transport routes, and a rational selection of means of transport, could positively influence both the perceived quality of care and the quality of drugs by the user. In addition, we study how to reduce energy consumption, the carbon footprint (CO2 ), the risk of non-supply, refrigeration, and monitoring costs, and, ultimately, the final cost of centralized drug distribution. On this basis, we have built a model taking all the data from the geographic locations of more than 2,500 pharmacies in the Community of Madrid, and with the help of the R program, the data have been classified into ‘clusters’ using minimum distances and giving different center solutions depending on the number of clusters.

Content Details:-

Corresponding author: Javier Espina Hellín*

Full Length Article: Optimizing Drug Distribution Logistics in the Community of Madrid

Journal: Journal of Family Medicine

Disclaimer: This content is not owned or created by us. It has been sourced from the respective site, Austin Publishing Group, and is intended purely for study and educational purposes. All rights belong to the original authors and publishers.

Effect of Different Environmental Exposures on Retinal Arteriolar and Venular Diameters in Healthy Individuals and Those with Increased Cardiovascular Risk: A Systematic Review

Context: Fundus scanning has emerged as a non-invasive way to estimate the risk for cardiovascular diseases. Research suggests that environmental risk factors, such as diet and smoking, influences the structure of the retinal microvasculature, particularly the central retinal arteriolar and venular equivalents (CRAE & CRVE). Objective: This systematic review provides an updated overview of existing research on the effect of diet, smoking, physical activity, air pollution, alcohol consumption and miscellaneous exposures on retinal microvasculature in healthy individuals and people at risk for cardiovascular disease. Data Sources: The databases PubMed, Web of Science, OVID were used as data sources and were last accessed September 12nd 2024. Study Selection: Peer-reviewed studies investigating the effect of any type of environmental exposure on retinal microvessel diameters in healthy individuals and people at risk for cardiovascular disease were included. Data Extraction: Relevant information and outcomes of 88 studies were extracted. Methodological quality was assessed using the NIH quality assessment tool. Data from each included study was extracted by two independent reviewers, while a third reviewer checked for consensus. Conclusions: Several dietary components, including numerous phytochemicals or fish oils have beneficial effects while other components (high glycemic index products, red meat) have deleterious effects on the retinal microvasculature. There was no unilateral association between smoking and CRAE nor CRVE. A decrease in retinal vessel diameters is induced by isometric exercise, whereas endurance exercise causes increased diameters. Fine Particulate Matter (PM2.5) exposure induces decreases in CRAE and CRVE. Further research is necessary to attain scientific consensus on the effects of alcohol consumption on the retinal microvasculature.  

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Corresponding author: Simone G Van Breda*

Full Length Article: Effect of Different Environmental Exposures on Retinal Arteriolar and Venular Diameters in Healthy Individuals and Those with Increased Cardiovascular Risk: A Systematic Review

Journal: Journal of Ophthalmology and Visual Sciences

Disclaimer: This content is not owned or created by us. It has been sourced from the respective site, Austin Publishing Group, and is intended purely for study and educational purposes. All rights belong to the original authors and publishers.

 

High-performance Liquid Chromatography Method Development and Validation for the Determination of Ellagic Acid in Topical Cream Formulations as Hyperpigmentation Treatment

Skin hyperpigmentation is a dermatological condition that cause a change in skin coloration. In this condition an overproduction of melanin happens in skin melanocytes. Ellagic Acid (EA) is a pharmaceutical substance that found in many fruits and plants which is quite useful to apply in skin products due to its antityrosinase and antioxidant activity. In this study a topical cream of EA was prepared and methanol was employed as extraction solvent to determine the amount of EA in the formulation. For this aim we have developed and validated a High-Performance Liquid Chromatography (HPLC) method in order to evaluation the amount of EA in cream. The developed method was validated against criteria such as linearity, specificity, precision, accuracy, Limit of Detection (LOD) and Limit of Quantification (LOQ) due to the International Council for Harmonization (ICH) guidelines, Q2. Our results showed high specificity, linearity (R2=0.9973), sensitivity and acceptable precision and accuracy rate. By our developed HPLC method the concentration of EA in topical cream was calculated, and it was found to be 9.81 mg/ml. Also, this method can be used for EA determining in lipid nanoparticles, such as liposomes, solid lipid nanoparticles (SLNs) and Nanostructured Lipid Carriers (NLCs)For more information, visit the full study through below link.

Content Details:-

Corresponding author: Golmohammadzadeh S, and Jaafari MR*

Full Length Article: High-performance Liquid Chromatography Method Development and Validation for the Determination of Ellagic Acid in Topical Cream Formulations as Hyperpigmentation Treatment

Journal: Austin Journal of Analytical and Pharmaceutical Chemistry

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Knuckle-Dimple-Dimple-Dimple Sign

 

A 15-year-old girl, second in birth order from consanguineous marriage, presented with progressive weight gain over five years. Upon examination, she had a round face, almond-shaped eyes, acanthosis nigricans, short stature (height SDS -2.5), and obesity (BMI 30.6 kg/m). Her hands and feet showed short, stubby fingers with positive Archibald sign, characterized by a “knuckle-dimple-dimple-dimple” pattern (Figure 1a). Her mother and maternal grandmother shared similar hand features, suggesting a familial inheritance. Radiographic evaluation of the hands revealed Type E brachydactyly based on the Tamtamy classification, with shortening of 3rd, 4th and 5th metacarpals and phalanges (Figure 1b). Biochemical testing showed normal calcium, phosphate, and Parathyroid Hormone (PTH) levels, ruling out true hypoparathyroidism. In presence of normal regulation of mineral ion homeostasis, short stature, obesity and typical Albright’s Hereditary Osteodystrophy (AHO) feature, diagnosis of pseudopseudo hypoparathyroidism was stablished. This rare autosomal dominant disorder is caused by GNAS gene mutation.

For more information, visit the full study through below link.

Content Details:-

Corresponding author: Ejaz Alam*

Full Length Article: Knuckle-Dimple-Dimple-Dimple Sign

Journal: Journal of Pediatric Endocrinology

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Advancing Epilepsy Diagnosis: Insights from EEG Signal Analysis with SVM and CNN

Epilepsy is a common neurological disorder in elderly populations, often linked to age-related conditions such as stroke and neurodegeneration. Traditional EEG signal analysis for epilepsy diagnosis is time-consuming, subjective, and unsuitable for clinical use, emphasizing the necessity for automated approaches. This study evaluates the performance of Support Vector Machine (SVM) and Convolutional Neural Network (CNN) in classifying EEG signals for epileptic seizure detection, utilizing the Bonn University EEG dataset. Time- and frequency-domain features were extracted, and 10-fold cross-validation was employed to validate the results. The findings reveal that SVM achieved 100% accuracy in distinguishing simple EEG states, such as healthy versus seizure conditions. Meanwhile, CNN outperformed SVM in processing more complex signals, achieving an average accuracy of over 98%. The results highlight the potential of integrating traditional machine learning with deep learning methods to enhance diagnostic accuracy and efficiency. These findings lay a strong foundation for developing advanced EEG-based diagnostic tools tailored to elderly epilepsy patients, facilitating more timely and effective clinical interventions

For more information, visit the full study through below link.

Content Details:-

Corresponding author: Ting Wang*

Full Length Article: Advancing Epilepsy Diagnosis: Insights from EEG Signal Analysis with SVM and CNN

Journal: Gerontology & Geriatrics: Research

Disclaimer: This content is not owned or created by us. It has been sourced from the respective site, Austin Publishing Group, and is intended purely for study and educational purposes. All rights belong to the original authors and publishers.