High-performance Liquid Chromatography Method Development and Validation for the Determination of Ellagic Acid in Topical Cream Formulations as Hyperpigmentation Treatment

Skin hyperpigmentation is a dermatological condition that cause a change in skin coloration. In this condition an overproduction of melanin happens in skin melanocytes. Ellagic Acid (EA) is a pharmaceutical substance that found in many fruits and plants which is quite useful to apply in skin products due to its antityrosinase and antioxidant activity. In this study a topical cream of EA was prepared and methanol was employed as extraction solvent to determine the amount of EA in the formulation. For this aim we have developed and validated a High-Performance Liquid Chromatography (HPLC) method in order to evaluation the amount of EA in cream. The developed method was validated against criteria such as linearity, specificity, precision, accuracy, Limit of Detection (LOD) and Limit of Quantification (LOQ) due to the International Council for Harmonization (ICH) guidelines, Q2. Our results showed high specificity, linearity (R2=0.9973), sensitivity and acceptable precision and accuracy rate. By our developed HPLC method the concentration of EA in topical cream was calculated, and it was found to be 9.81 mg/ml. Also, this method can be used for EA determining in lipid nanoparticles, such as liposomes, solid lipid nanoparticles (SLNs) and Nanostructured Lipid Carriers (NLCs)For more information, visit the full study through below link.

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Corresponding author: Golmohammadzadeh S, and Jaafari MR*

Full Length Article: High-performance Liquid Chromatography Method Development and Validation for the Determination of Ellagic Acid in Topical Cream Formulations as Hyperpigmentation Treatment

Journal: Austin Journal of Analytical and Pharmaceutical Chemistry

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